Composite

Part:BBa_K2121005:Design

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


Taurine Inducible Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 471
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4905
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 471
    Illegal NotI site found at 2779
    Illegal NotI site found at 4312
    Illegal NotI site found at 5602
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1487
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 471
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 471
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 2224
    Illegal NgoMIV site found at 2398
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 2452
    Illegal NgoMIV site found at 2479
    Illegal NgoMIV site found at 2978
    Illegal AgeI site found at 92
    Illegal AgeI site found at 5399
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5993
    Illegal SapI.rc site found at 5530


Design Notes

Utilize native suppression mechanism in S. meliloti.

Construct design Design_1.png

Design_2.png



Source

Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.

References